Iliev Lab

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Research

Immunity to Mucosa-Associated Fungi

Diverse fungal communities (mycobiomes) colonize all mucosal surfaces of the body. While some commensal fungi can be opportunistic pathogens and can lead to serious infections upon a bridge of the mucosal barriers, evidence coming from the Iliev Laboratory suggests that a balanced fungal community is necessary for the maintenance of intestinal health.

The Iliev Laboratory is specifically interested in molecular mechanisms by which fungi interact with gut-resident phagocytes and polarize protective adaptive immune responses. Using in vivo murine models lacking immune arms and antifungal receptors, we study the interaction of commensal fungi with the gut immune system during steady state and during colitis. Our final goal is to understand how this interaction is regulated and provide strategies to manipulate these pathways.

Mucosal immune responses to commensal fungi in the intestine.


Characterization of Intestinal Fungal Communities (Mycobiota) and Their Interaction with Bacteria

Fungal and bacterial communities share similar niches in the intestine where they interact and co-depend on each other. Using murine models of intestinal inflammation and samples from patients with inflammatory bowel disease, the Iliev Lab aims to understand the interconnection between gut fungal and bacterial communities. We study how changes in bacterial communities will affect the fungal counterpart of the intestinal microbiota and whether bacteria can respond to changes in the mycobiota. The lab utilizes a combination of approaches to promote microbiota instability in vivo  and to track pathways important for the interaction between fungi and bacteria. We aim to address a fundamental question about how eukaryotic and prokaryotic microbes interact in the intestine.

Commensal fungi (green) cohabitate with commensal bacteria (red) in the mouse gut

Gut mycobiota and inflammation

Inflammatory bowel disease (IBD) is a complex disease, driven by a combination of genetic and environmental factors leading to immune responses against a host’s own microbiota. We have recently shown that innate immune deficiency in the antifungal receptor Dectin-1 predisposes murine models to intestinal inflammation triggered by gut fungi. A polymorphism of CLEC7a, the gene encoding for Dectin-1, predisposes patients to severe ulcerative colitis (a form of IBD). Recent clinical and experimental studies suggest that microbiota composition dramatically changes in the course of the intestinal inflammation. We apply microbiota transplantation, microbiota modification with drugs and several murine models of intestinal inflammation to assess the role of the gut mycobiota in disease development and progression. Bacterial and fungal changes during the course of disease are analyzed by using nextgen sequencing approaches and microbiota analysis platforms.

 

Weill Cornell Medicine Iliev Lab 413 E 69th Street, BB-752 New York, NY 10021 Phone: (646) 962-7236